If microscopy methods other than bright field observation are used

-the case of phase contrast observation-

Basics

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

To conduct phase contrast observation, perform the following steps after completing procedures 1, 2, 3 and 4, which are explained in the Basics section.

 

 

 

 

 

 

 

 

Set the aperture iris diaphragm to the fully open position.

Switch to the phase contrast objective of your choice.

Set the condenser turret to an appropriate position on the condenser ring slits (Ph1, Ph2, etc.) specified for the phase contrast objective to be used.

Remove one of the two eyepieces, and install the centering telescope.

Adjust the focus of the centering telescope in such a way that the condenser ring slit (bright ring) and objective phase plate (dark ring) become clearly visible.

By manipulating the condenser ring slit centering screws of the condenser, align the condenser ring slit and objective phase plate concentrically.

 

 

 

 

 

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