| If microscopy methods other than bright field observation are used -the case of phase contrast observation- | Basics |

|  | To conduct phase contrast observation, perform the following steps after completing procedures 1, 2, 3 and 4, which are explained in the Basics section. |  |
| Set the aperture iris diaphragm to the fully open position.
| Switch to the phase contrast objective of your choice.
| Set the condenser turret to an appropriate position on the condenser ring slits (Ph1, Ph2, etc.) specified for the phase contrast objective to be used.
| Remove one of the two eyepieces, and install the centering telescope.
| Adjust the focus of the centering telescope in such a way that the condenser ring slit (bright ring) and objective phase plate (dark ring) become clearly visible. |
| By manipulating the condenser ring slit centering screws of the condenser, align the condenser ring slit and objective phase plate concentrically.
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