| If microscopy methods other than bright field observation are used - the case of DIC observation - (1) | Basics |

|  | To conduct DIC observation, perform the following steps after completing procedures 1, 2, 3 and 4, which are explained in the Basics section. |  |
| Remove any specimens.
| Set the condenser turret to the “BF” position.
| Pull the DIC slider out.
| Push the polarizer all the way in.
| Push the analyzer until it clicks into position.
| Remove the eyepiece and look into the eyepiece sleeve.
| Turn the polarizer rotation knob; stop turning it at the position where it becomes darkest.
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