4. Software for Laser Scanning Microscopes
Images captured from laser scanning microscopes can be processed and analyzed using computer software as a final step to obtain the desired output. This software is also an important element that determines the operability of the device and compatibility with samples. The following is a brief introduction to some of the imaging, analysis, and display functions supported by this software:
4-1. Imaging
Captured images are usually preprocessed for analysis or display. Image processing generates an image for display that is easy to observe and facilitates analysis. Inclination correction; smoothing filter, emphasis filter, and frequency filter, among other filters; isolated point removal; contour correction; and other functions can be used to eliminate unnecessary noise.
4-2. Analysis (Measurement))
The status of the sample surface can be digitized and used for quantitative determination. Generally, a variety of analysis software applications are included, for example, applications for profile analysis, line width measurement, roughness analysis, area and volume measurement, particle analysis, geometrical measurement, and coating thickness measurement. Recently, use of the microscope as a noncontact face roughness measuring unit has increased.
4-3. Display
A variety of display methods can be used to visually recognize the shape of a sample. Three-dimensional display supports the line method, mesh method, surface method, shadow method, contrast/shadow method, and the intensity/height method, which is characteristic of the laser scanning microscope. In addition, samples can be observed at a high resolution and in wide field by stitching several images together. Using these software applications to meet a variety of requests can be expected to further widen the application range of laser scanning microscopes in the future.
- 1. Basic Principles of Laser Scanning Microscopes
- 2. Resolving Power of Laser Scanning Microscopes
- 3. Measurement Accuracy of Laser Scanning Microscopes
- 4. Software for Laser Scanning Microscopes
- 5. Basic Features of Olympus Industrial Laser Scanning Microscopes
- 6. Application Examples
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